Journal: AMB Express

Article Title: Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation

doi: 10.1186/s13568-024-01736-x

Figure Lengend Snippet: Inhibition effects of Coptis rhizome ethanol extract and water extract on two S. pneumoniae strains ( A , ATCC ATCC 49619; B , ATCC 70067), showcasing respective inhibition zone diameters. Comparative visualization of antibacterial efficacy through the size of inhibition zones (mm) for each strain and extract. Rifampin (5 µg) was positive control. The data was displayed as the mean ± standard error of the mean (SEM). CRET30, Coptis rhizome extracted with ethanol 30%; CRET50, Coptis rhizome extracted with ethanol 50%; CRET70, Coptis rhizome extracted with ethanol 70%; CRDW, Coptis rhizome extracted with distilled water

Article Snippet: Fig. 1 Inhibition effects of Coptis rhizome ethanol extract and water extract on two S. pneumoniae strains ( A , ATCC ATCC 49619; B , ATCC 70067), showcasing respective inhibition zone diameters.

Techniques: Inhibition, Positive Control

Journal: AMB Express

Article Title: Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation

doi: 10.1186/s13568-024-01736-x

Figure Lengend Snippet: Minimum Inhibitory Concentration (MIC) results for Coptis rhizome extracted with either different concentration of ethanol or water against S. pneumoniae strains ATCC 49619 ( A ) and ATCC 70067 ( B ). CRET30, Coptis rhizome extracted with ethanol 30%; CRET50, Coptis rhizome extracted with ethanol 50%; CRET70, Coptis rhizome extracted with ethanol 70%; CRDW, Coptis rhizome extracted with distilled water

Article Snippet: Fig. 1 Inhibition effects of Coptis rhizome ethanol extract and water extract on two S. pneumoniae strains ( A , ATCC ATCC 49619; B , ATCC 70067), showcasing respective inhibition zone diameters.

Techniques: Concentration Assay

Journal: AMB Express

Article Title: Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation

doi: 10.1186/s13568-024-01736-x

Figure Lengend Snippet: Time-kill curves of Coptis rhizome extracted with ethanol 70% at different concentration (1MIC = 0.0625 μg/mL, 2MIC = 0.125 μg/mL, 4MIC = 0.25 μg/mL), or left untreated as a control (CTRL) against S. pneumoniae strains ATCC 49619 ( A ) and ATCC 70067 ( B ). Rifampin is regarded as the positive control for comparison. The data was displayed as the mean ± standard error of the mean (SEM)

Article Snippet: Fig. 1 Inhibition effects of Coptis rhizome ethanol extract and water extract on two S. pneumoniae strains ( A , ATCC ATCC 49619; B , ATCC 70067), showcasing respective inhibition zone diameters.

Techniques: Concentration Assay, Control, Positive Control, Comparison

Journal: AMB Express

Article Title: Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation

doi: 10.1186/s13568-024-01736-x

Figure Lengend Snippet: Bacterial lysis following treatment with Coptis rhizome extracted with ethanol 70% at different concentration (1MIC = 0.0625 μg/mL, 2MIC = 0.125 μg/mL, 4MIC = 0.25 μg/mL) or left untreated as a control (CTRL) against S. pneumoniae strains ATCC 49619 ( A ) and ATCC 70067 ( B ). OD values at 595nm was depicted. The data was displayed as the mean ± standard error of the mean (SEM)

Article Snippet: Fig. 1 Inhibition effects of Coptis rhizome ethanol extract and water extract on two S. pneumoniae strains ( A , ATCC ATCC 49619; B , ATCC 70067), showcasing respective inhibition zone diameters.

Techniques: Lysis, Concentration Assay, Control

Journal: AMB Express

Article Title: Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation

doi: 10.1186/s13568-024-01736-x

Figure Lengend Snippet: Inhibition of biofilm (%) by the bacteria S. pneumoniae ATCC 49619 after treating Coptis rhizome extract . The data was displayed as the mean ± standard error of the mean (SEM). CRET30, Coptis rhizome extracted with ethanol 30%; CRET50, Coptis rhizome extracted with ethanol 50%; CRET70, Coptis rhizome extracted with ethanol 70%; CRDW, Coptis rhizome extracted with distilled water.

Article Snippet: Fig. 1 Inhibition effects of Coptis rhizome ethanol extract and water extract on two S. pneumoniae strains ( A , ATCC ATCC 49619; B , ATCC 70067), showcasing respective inhibition zone diameters.

Techniques: Inhibition, Bacteria

Journal: AMB Express

Article Title: Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation

doi: 10.1186/s13568-024-01736-x

Figure Lengend Snippet: Expression levels of the lytA gene in S. pneumoniae strains: standard ATCC 49619 ( A ) and resistant ATCC 70067 ( B ). Analysis of autolysin production under Coptis rhizome extracted with ethanol 70% (CRET70) concentrations: 1MIC, 2MIC, 4MIC. The group not treated with the test compound served as the control (CTRL). The values shown were the average differences in expression fold change compared to cells that were not treated. The data was displayed as the mean ± standard error of the mean (SEM) and is based on three separate experiments. * P < 0.05, ** P < 0.01,*** P < 0.001

Article Snippet: Fig. 1 Inhibition effects of Coptis rhizome ethanol extract and water extract on two S. pneumoniae strains ( A , ATCC ATCC 49619; B , ATCC 70067), showcasing respective inhibition zone diameters.

Techniques: Expressing, Control